List of GCPLab SOPs
| SOP Number | SOP Title | Download | |||
| GCLPEQU001
| Use of an ESCO Airstream Class II Biological Safety Cabinet | ||||
| GCLPEQU002
| Use of the Pharmagraph EMS | ||||
| GCLPEQU003
| Responding to a Pharmagraph SMS Alert | ||||
| GCLPEQU004
| Use of Pipettes | ||||
| GCLPEQU005
| Testing and Calibration of Pipettes | ||||
| GCLPEQU006
| Use of the Chemometec Nucleocounter | ||||
| GCLPEQU007
| Use of a Grant Waterbath | ||||
| GCLPEQU008
| Use of a Sensitech Temptale 4 Monitor | ||||
| GCLPEQU009
| Calibration of the Chemometic Nucleocounter | ||||
| GCLPEQU010
| Use of the Heraeus 3SR+ Refrigerated Bench Top Centrifuge | ||||
| GCLPEQU011
| Use of Sartorius Balances (TE214S, TE1502S and TE6100) | ||||
| GCLPEQU015
| Use of a Haemocytometer | ||||
| GCLPEQU016
| Use of the Bio Rad Bio-Plex 200 Instrument and Bio-Plex Manager Software Version 5.0 (Luminex) | ||||
| GCLPEQU017
| Use of the Wellwash 4 Mk2 Microplate Washer | ||||
| GCLPEQU018
| Use of the Multiskan EX Microplate Reader | ||||
| GCLPEQU019
| PH Meter | ||||
| GCLPEQU020
| Use of the MagNA Pure | ||||
| GCLPEQU021
| Using the Leica RM2235 Microtome to Cut Paraffin Embedded Tissue Blocks | ||||
| GCLPEQU022
| Use of the Ion Torrent | ||||
| GCLPEQU023
| Ion Torrent: Cleaning and Shutdown | ||||
| GCLPEQU024
| Use of the Bio-Plex Pro Wash Station | ||||
| GCLPEQU025
| Use of the Sigma 3-16PK Refrigerated Centrifuge | ||||
| GCLPEQU026
| Use of a SafeFAST Top Class II Biological Safety Cabinet | ||||
| GCLPEQU027
| Defrosting Ultra Low Temperature Freezers | ||||
| GCLPEQU028
| FACS BD LSRFortessa Daily Start Up and Shutdown | ||||
| GCLPEQU029
| Use of the NanoDrop for Quantification of Protein, RNA and DNA | ||||
| GCLPEQU030
| Use of Pipettes for Cell Sorting Facility | ||||
| GCLPEQU031
| Using a Sonicating Bath for Cell Sorting Facility | ||||
| GCLPEQU032
| Testing and Calibration of Pipettes for Cell Sorting Facility | ||||
| GCLPEQU033
| Monitoring of Refrigerator Temperature for Cell Sorting Facility | ||||
| GCLPEQU035
| Use of Magnesium Assay Kit in the GCLP Facility | ||||
| GCLPEQU036
| Use of Cryostat in the GCLP Facility | ||||
| GCLPEQU037
| Use of Pipettes in the PBRU | ||||
| GCLPEQU038
| Use of Centrifuges in the PBRU | ||||
| GCLPEQU039
| Use of the Mettler Toledo S20 pH Meter in the PBRU | ||||
| GCLPEQU040
| Use of PBRU Biological Safety Cabinets | ||||
| GCLPEQU041
| Use of the Maxwell RSC Instrument | ||||
| GCLPEQU042
| Use of the Quantus™ Fluorometer | ||||
| GCLPEQU043
| Out of Use Equipment | ||||
| GCLPEQU044
| Non-GCP and Out of Use Equipment for Cell Sorting Facility | ||||
| GCLPEQU045
| Maintenance of the Daytona | ||||
| GCLPEQU046
| Management of the Pharmagraph EMS | ||||
| GCLPFAC001
| Production, Control, Review, Update and Withdrawal of SOPs and Documents for the GCP Facility | ||||
| GCLPFAC002
| Induction of Staff New to the GCP Facility | ||||
| GCLPFAC003
| Performing a Stock Check | ||||
| GCLPFAC004
| Dealing with Inappropriately, Inadequately, Incorrectly Labelled, Late Samples or Samples Received in Error | ||||
| GCLPFAC005
| Dealing with Disaster Situations | ||||
| GCLPFAC006
| Induction of Staff New to the GCLP Facility for Cell Sorting Facility | ||||
| GCLPFAC007
| Dealing with Disaster Situations for Cell Sorting Facility | ||||
| GCLPFAC008
| Training of Staff on the AP Biobank Rota | ||||
| GCLPFAC009
| Computer Hardware and Software Tracking and Validation | ||||
| GCLPFAC010
| Archiving Data and Information | ||||
| GCLPFAC011
| Obtaining & Managing Informed Consent for the LPRG Acute Pancreatitis Research Tissue Bank | ||||
| GCLPFAC012
| Good Documentation Practice | ||||
| GCLPQAU002
| Conduct of Audits | ||||
| GCLPRPS001
| Haematoxylin and Eosin Staining of Paraffin Embedded Tissue Sections on Glass Microscope Slides | ||||
| GCLPRPS002
| Opening, Storing and Disposal of Reagents and Solutions | ||||
| GCLPRPS003
| Parallel Testing of New Reagents | ||||
| GCLPRPS004
| Disposal of Hazardous Waste | ||||
| GCLPRPS006
| Use and Disposal of 3H(Tritium) in the GCLP Facility | ||||
| GCLPRPS007
| The Receipt and Storage of Frozen Samples from Site | ||||
| GCLPRPS008
| Writing COSHH Assessments | ||||
| GCLPRPS009
| M30 ELISA for Measurement of a Circulating Caspase-Cleaved Form of Cytokeratin 18 | ||||
| GCLPRPS010
| M65 ELISA for Measurement of Total Circluating Cytokeratin 18 | ||||
| GCLPRPS011
| Use of the LIMS to Record Kit Construction, QC, Storage and Dispatch | ||||
| GCLPRPS013
| Use of Freezers | ||||
| GCLPRPS014
| Receipt of Samples | ||||
| GCLPRPS015
| Construction of Tissue MicroArrays | ||||
| GCLPRPS016
| Embedding of Tissue in Paraffin Wax | ||||
| GCLPRPS018
| Writing COSHH Assessments for Cell Sorting Facility | ||||
| GCLPRPS019
| Opening, Storage and Disposal of Reagents and Solutions for Cell Sorting Facility | ||||
| GCLPRPS020
| Disposal of Hazardous Waste for Cell Sorting Facility | ||||
| GCLPRPS022
| Finding Available Freezer Space Using the LIMS | ||||
| GCLPRPS024
| Disposal of Hazardous Waste in the PBRU | ||||
| GCLPRPS026
| Close Down of the Randox RX Daytona Clinical Analyser | ||||
| GCLPRPS027
| Calibration of the RX Daytona Clinical Analyser | ||||
| GCLPRPS029
| Use of the Randox Magnesium Assay for the RX Daytona Clinical Analyser | ||||
| GCLPRPS030
| Start Up of the Randox RX Daytona Clinical Analyser | ||||
| GCLPRPS032
| Reagent Labeling and Expiry | ||||
| GCLPRPS033
| KIM-1 Assay on the Bio-Plex | ||||
| GCLPRPS034
| Creatinine Assay | ||||
| GCLPTSS006
| Inactivation and Storage of AB Human Serum | ||||
| GCLPTSS008
| Extraction of Blood Plasma and Cell Pellet from HOPON Trials | ||||
| GCLPTSS010
| Preparation of Haematology and Oncology Kits | ||||
| GCLPTSS011
| Assembly of Kits for the ESPAC-4 Trial | ||||
| GCLPTSS012
| Sample Collection, Processing and Storage for the Secretin Study | ||||
| GCLPTSS017
| Generic Procedure for Sample Processing for SNP Assays | ||||
| GCLPTSS018
| Processing Anti-Coagulated Blood for PBMCs and Plasma Isolation and Storage for the UK CLL Trials Biobank | ||||
| GCLPTSS019
| Use of The Perkin Elmer Microbeta Counter | ||||
| GCLPTSS020
| Use of the Tomtec Cell Harvester | ||||
| GCLPTSS022
| Processing Coagulated Blood for Serum Isolation & Storage for the UK CLL Trials | ||||
| GCLPTSS023
| The Reception & Processing of ESPAC-4 Samples from External Sites & from the Royal Liverpool University Hospital | ||||
| GCLPTSS024
| Processing of Frozen Samples for the ESPAC-4 Trial | ||||
| GCLPTSS029
| Processing PACIFICO Sequential Anticoagulated Blood Samples | ||||
| GCLPTSS032
| Receipt of Clinical Trials Kit Boxes for PACIFICO and UK CLL Trials | ||||
| GCLPTSS034
| EUROPAC2 Samples: Kit Preparation, Distribution and Receipt | ||||
| GCLPTSS036
| Processing Bone Marrow for UK CLL Trials Biobank | ||||
| GCLPTSS038
| Chronic Lymphocytic Leukaemia (CLL) Minimal Residual Disease Detection by Multicolor Flow Cytometry | ||||
| GCLPTSS039
| Use of Spare Kits in the Event of a Mislaid Processing Part of a Kit | ||||
| GCLPTSS040
| Processing of Samples for the PBRU Chronic Pancreatitis Biobank and Controls Biobank | Download | |||
| GCLPTSS041
| Programming and Downloading Escort iMini Data Loggers | ||||
| GCLPTSS045
| Sending Out UK CLL Trials Biobank Sample Kits from the GCLP Laboratory | ||||
| GCLPTSS046
| Kit Preparation for the Pancreas Bank | Download | |||
| GCLPTSS047
| Sample Collection, Reception and Tissue Storage for the Pancreas Bank | Download | |||
| GCLPTSS048
| Processing of Samples for the Pancreas Bank | Download | |||
| GCLPTSS049
| Processing of Samples for the PBRU Acute Pancreatitis Biobank | Download | |||
| GCLPTSS055
| Collection of Samples for the Chronic Pancreatitis Biobank | Download | |||
| GCLPTSS059
| FACS BD Canto II Daily Start Up and Shutdown | ||||
| GCLPTSS060
| Setting Up a New Experiment and Counting on FACS | ||||
| GCLPTSS061
| Analysing CLL MRD Data for Reporting | ||||
| GCLPTSS068
| Receipt of Central Pathology Review Samples to the GCLP Laboratories | ||||
| GCLPTSS069
| Forwarding Samples to the Liverpool Women | ||||
| GCLPTSS070
| Preparing Cell Pellets from Blood Samples and Making Blood Films | ||||
| GCLPTSS073
| EUROPAC Sample Processing Lymphocyte Preparation and Storage | ||||
| GCLPTSS074
| Transfer of Bone Marrow Trephine to Pathology Department | ||||
| GCLPTSS084
| Transfer of Frozen Samples From Long Term Storage in the GCLP Facility to Long Term Storage in Another University Facility | ||||
| GCLPTSS085
| Multicolour Flow Cyctometric Analysis of Allogeneic Celll Immunotherapy (ACIT-1) Surface Markers | ||||
| GCLPTSS086
| Taking, Storing and Dispatching FFPE Tissue Block Sections for the PACIFICO Trial | ||||
| GCLPTSS087
| Setting Colour Compensation for Flow Cytometric Analysis | ||||
| GCLPTSS088
| Reporting of Laboratory Quality Incidents on the Matrix LIMS | ||||
| GCLPTSS089
| Isolation of Genomic DNA from Saliva Samples for the CLL Trials Biobank | ||||
| GCLPTSS093
| PCR Analysis of Allogenic Cell Immunotherapy (ACIT-1) Antigen Expression | ||||
| GCLPTSS094
| Quantatative Detection of Allogenic Cell Immunotherapy (ACIT-1) Telomerase Expression | ||||
| GCLPTSS096
| FACS BD Canto II Daily Start Up and Shutdown for Cell Sorting Facility | ||||
| GCLPTSS097
| Setting Up a New Experiment and Counting on FACS for Cell Sorting Facility | ||||
| GCLPTSS098
| Setting Colour Compensation for Flow Cytometric Analysis for Cell Sorting Facility | ||||
| GCLPTSS100
| FACS Aria Start Up and Shutdown for the Cell Sorting Facility | ||||
| GCLPTSS101
| FACS Aria III Maintenance Procedure for Cell Sorting Facility | ||||
| GCLPTSS103
| Experimental Setup for the BD FACS ARIA III for Cell Sorting Facility | ||||
| GCLPTSS105
| Processing and Storing Blood for the LIVC3 Study | ||||
| GCLPTSS106
| DNA Extraction and Quantification from EDTA Blood Pellets for the ViP Trial | ||||
| GCLPTSS108
| RET SNP Analysis of ViP DNA Samples Using the Roache Lightcycler 480 | ||||
| GCLPTSS110
| Preparation of Kits for the ESPAC-5FT Study | ||||
| GCLPTSS113
| Processing of Blood and Urine for the ESPAC-5FT Study | ||||
| GCLPTSS114
| Assembly of Kits for the NEO-BLADE Trial | ||||
| GCLPTSS116
| Collection of Samples for the Acute Pancreatitis Biobank in the PBRU | Download | |||
| GCLPTSS117
| Collection of Samples for the Control Biobank | ||||
| GCLPTSS118
| Isolation of Genomic DNA from Whole Blood for the PACIFICO Trial | ||||
| GCLPTSS120
| Central Pathology Review Reporting for CLL210 and RIAltO | ||||
| GCLPTSS121
| Multiple SNP Analysis and Validation of ViP DNA Samples Using the Roche LightCycler 480 | ||||
| GCLPTSS123
| Processing of Targovax Samples in the Liverpool GCP Lab Facility | ||||
| GCLPTSS124
| Receipt and Processing of Samples for the ACIT-1 Clinical Trial | ||||
| GCLPTSS125
| Transfer of ACIT-1 Vials from GCP Facility to Pharmacy | ||||
| GCLPTSS126
| Shipping Frozen ACIT-1 Samples to KingsPath, Kings College London | ||||
| GCLPTSS136
| Transfer of Vials from Mobile Liquid Nitrogen Container to -150oC Freezer for ACIT-1 | ||||
| GCLPTSS137
| Completion of Acute Pancreatitis Health Information Database | ||||
| GCLPTSS138
| Completion of Electronic Acute Pancreatitis Health Information Database | ||||
| GCLPTSS139
| Collection and Processing of Samples for the PancDiab Study | ||||
| GCLPTSS140
| Collection, Processing and Storage of PK-PBMC Acelarate Samples | ||||
| GCLPTSS141
| Preparation of Blood and Urine Kits for the CiPHER Study | ||||
| GCLPTSS142
| Processing and Storing Blood and Urine for the CiPHER Trial | ||||
| GCLPTSS143
| Processing and Storing of NICO Samples in the GCP Facility | ||||
| GCLPTSS148
| Obtaining Informed Consent for Participants for the Chronic Pancreatitis Research Tissue Bank | Download | |||
| GCLPTSS149
| Obtaining Informed Consent for Participants Recruited for the Control Biobank | ||||
| GCLPTSS152
| Sample Processing and Storage in the GCLP Facility for the PANasta Trial | ||||
| GCLPTSS153
| Processing and Storing of Acelarate Samples in the GCLP Facility | ||||
| GCLPTSS156
| Receipt Processing and Storage of BBC Translational Samples | ||||
| GCLPTSS157
| Locating and Requesting of PBRU Biobank Samples | ||||
| GCLPTSS159
| PBRU Disaster Recovery | Download | |||
| GCLPTSS160
| Management of CLL MRD Data | ||||
| GCLPTSS161
| Cell Counting in the PBRU | ||||
| GCLPTSS162
| Bioenegetic Profiling of Leukocytes in Peripheral Blood from PBRU | ||||
| GCLPTSS166
| Completion of the Chronic Pancreatitis Database Forms | ||||
| GCLPTSS167
| Completion of the Electronic Pancreatitis Database for the Chronic Pancreatitis Biobank | ||||
| GCLPTSS168
| Completion of the Control Database Proforma | ||||
| GCLPTSS169
| Completion of the Electronic Control Biobank Database | ||||
| GCLPTSS170
| Processing and Storing of PATHOS Samples in the GCP Facility | ||||
| GCLPTSS174
| Receipt and Storage of Samples at the Liverpool GCP Facility for SelPac Clinical Trial | ||||
| GCLPTSS175
| Processing Samples for the ReBeL Trial | ||||
| GCLPTSS176
| Isolation and Freezing of PBMCs for the NOPRODHPB0003 Study | ||||
| GCLPTSS177
| Receipt, Storage and Dispatch of EMERALD Trial Samples for the GCP Laboratory | ||||
| GCLPTSS178
| Cytof Phenotyping of Leukocytes from the LPRG Acute Pancreatitis Biobank | ||||
| GCLPTSS179
| Logging Receipt of Biobanking Samples to the UK CLL Trials Biobank | ||||
| GCLPTSS180
| Processing Cell Free DNA Tubes for the UK CLL Trials Biobank | ||||
| GCLPTSS181
| Receipt, Storage, Aliquoting & Distribution of the Androgen Recept Antibody for the EMERALD Trial | ||||
| GCLPTSS182
| Extraction of DNA/RNA for the ESPAC-T, ESPAC-4 & ESPAC-5 Clinical Trials | ||||
| GCLPTSS183
| Collection, Processing and Storage of Samples for the IMMPACT Study | ||||
| GCLPTSS184
| Collection & Processing of Disease & Healthy Control Samples for Costello Study 207897 | ||||
| GCLPTSS185
| Processing of Samples for the COMICE Trial | ||||
| GCLPTSS186
| Processing of VAC18194RSV2001 Samples | ||||
| GCLPTSS188
| Processing Samples for the PEARL Trial in the GCP Lab | ||||
| GCLPTSS190
| Processing and Storing Samples for the TACE-3 Trial | ||||
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